This study presents the complete genome sequence of a novel nege-like virus identified in whiteflies (Bemisia tabaci MEAM1), provisionally designated as whitefly negevirus 1 (WfNgV1). The virus possesses a single-stranded RNA genome comprising 11,848 nucleotides, organized into four open reading frames (ORFs). These ORFs encode the putative RNA-dependent-RNA-polymerase (RdRp, ORF 1), a glycoprotein (ORF 2), a structural protein with homology to those in the SP24 family, (ORF 3), and a protein of unknown function (ORF 4). Phylogenetic analysis focusing on RdRp and SP24 amino acid sequences revealed a close relationship between WfNgV1 and Bemisia tabaci negevirus 1, a negevirus sequence recently discovered in whiteflies from Israel. Both viruses form a clade sharing a most recent common ancestor with the proposed nelorpivirus and centivirus taxa. The putative glycoprotein from ORF 2 and SP24 (ORF 3) of WfNgV1 exhibit the characteristic topologies previously reported for negevirus counterparts. This marks the first reported negevirus-like sequence from whiteflies in the Americas.

Purpose: The objective of the research was aimed at achieving the implementation of the theory of constraints on the operating conditions of a hotel, which differs by its characteristics of traditional processes that have applied this method, from the great heterogeneity of resources needed to meet the demand of customers.Design/methodology/approach: To achieve this purpose, a method of generating conversion equations that allowed to express all the resources of the organization under study depending on the number of customers to serve facilitating comparison between different resources and estimated demand through techniques developed traditional forecasting, these features were integrated into the classical methodology of theory of constraints.Findings: The application of tools designed for hospitality organizations allowed to demonstrate the applicability of the theory of constraints on entities under conditions different from the usual, develop a set of conversion equations of different resources facilitating comparison with demand and consequently achieve improve levels of efficiency and effectiveness of the organization.Originality/value: The originality of the research is summarized in the application of the theory of constraints in a very different from the usual conditions, covering 100% of the processes and resources in hospitality organizations.

Purpose: This investigation is based on the theoretical analysis of the application of neural networks to the design and manage supply chains, along with an empirical approach, this investigation its developed with the prediction of the level of integration in the supply chain through neural networks.Design/methodology/approach: The methodology designed and used for the processing of data was the instruction of a neural network wich is used to predict the level of integration in a supply chain. This type of predictive application appears in the literature reviewed on supply chains. This analysis was carried out in a comparative way with the heterogeneous and homogeneous weights of the neuron training.Findings: The main results of this research focus on predicting the level of integration in the supply chain from the neoronal network. This provides a coached neuron that can be applied in other studies and, therefore, predict the outcome. On the other hand, it is shown that if the weights of the integration level variables are not homogeneous, the procedure presents different results depending on the context in which it is developed.Research limitations/implications: Among the limitations of the implementation of neural networks it should be noted, the necessary adaptation to the characteristics of the supply chains and the areas of performance of the business organizations under study, in the framework of activities productive or service itself, in addition to analyzing its corporate purpose in relation to the satisfaction of certain needs of the target markets.Originality/value: The literature shows multiple theoretical sources that refer to studies of neural networks in supply chains, observing the opportunity to apply this technique to predict the level of integration due to its benefits for decision making. The originality of this scientific work lies in the possibility of comparing the historical data of the level of integration and those predicted as a result of the coaching of the neuron with the weights of the heterogeneous and homogeneous variables.

The purpose of this research work is to illustrate that using the logic of theory of constraints (TOC) concerning the Throughput is adequate in management decisions to improve performance in the context of small and medium enterprises. The TOC approach assumes the use of Throughput as an indicator of performance, and in this paper the method of chain substitution and successive approximation is proposed to analyze this indicator. After the implementation of the Throughput per hour analysis, the owner-manager changed her perspective about what her priorities should be in the exercise of the administrative process. The identification of the restriction of what prevented him from obtaining more money allowed him to make the decisions or courses of action that maximized the Throughput per hour, thus improving his performance as owner-manager, the performance of his enterprise and the economic situation of his employees. The originality of this article consists in the application of TOC approach through the design of the indicator Throughput per hour to analyse the performance of an entrepreneur and the application of the chain substitution and successive approximation method for the study of the restrictions that inhibit it.

The complete genome sequence of Alstroemeria necrotic streak virus (ANSV) isolated from the statice cut flower ( Limonium sinuatum ) is presented. The genome has the three characteristic segments of the genus Orthotospovirus , family Tospoviridae , the S segment (3,135 bp), the M segment (4,869 bp), and the L segment (8,755 bp).

In this work, we present the complete genome sequence of Lily symptomless virus isolated from Alstroemeria plants. The genome is 8,391-bp long, arranged into six open reading frames (ORFs), with a 5′-untranslated region (5′-UTR) of 55 nucleotides (nt) and a 3′-UTR of 48 nt.

The study provides a discussion about the leadership style of small businesses owner-managers at Santo Domingo de los Tsáchilas, Ecuador, supported on contingency theory of leadership. And adaptation of Hershey and Blanchard questionnaire was made in order to make it clear to the target population. A total of 175 owner-managers of small businesses and 1218 employees comprised the respondents of the study. The perception of leadership style of owner-managers and employees, style range, and style adaptability and perception of the maturity degree of employees were the variables involved in the hypotheses raised. In spite of totally or partially proving the hypothesis raised, findings of the study revealed mixed support to the theory which impedes giving clear recommendations about how to behave in certain organizational situations in the context of small Ecuadorian businesses. Results of the study caution owner-managers of small businesses to consider carefully the way they behave in certain situation to the enrichment of employee motivation toward work with the purpose to obtain a better performance of their enterprises; but should avoid assuming that there are more or less appropriate leader behaviors based on the results of this research. The study constitutes a starting point to focus the research related to leadership style in small companies, considering the preponderance of this type of business in the economy of Ecuador.